Tlr4 Knockout cell line (Raw 264.7)

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Tlr4 Knockout cell line (Raw 264.7) Order

Tlr4 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0005
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Tlr4 Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Tlr4 Knockout cell line (Raw 264.7)
Species
Gene Name
Tlr4
Gene ID
21898
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Tlr4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Tlr4 gene knockout Tlr4 Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Tlr4 Knockout cell line (Raw 264.7)
Tlr4 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0005
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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